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60s Clustered Heritage Boots in Black
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67,95 € *
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''These 60s Clustered Heritage Boots in Black are made for walking... and dancing... or even shopping!'' ;-) Diese vielseitigen Stiefeletten sind der perfekte Begleiter in dieser Jahreszeit! Hergestellt aus schwarzem Leder-Imitat mit Schnürsenkeln, geflochtenen Details und einem hohen Blockabsatz für den perfekten Vintage-Look.''Boot season is here!'' * Leder-Imitat * Runde Schuhspitze * Geflochtene Details * Hoher Blockabsatz * Schnürsenkel * Komfortables Fußbett * Anti-rutsch Sohle

Anbieter: Topvintage
Stand: 18.09.2020
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Characterization of a haloarchaeal 16S rRNA gen...
40,90 CHF *
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Diploma Thesis from the year 2002 in the subject Biology - Micro- and Molecular Biology, grade: 1,0 (A), University of Salzburg (Institute for Genetics and General Biology), language: English, abstract: Using a new method to extract total community DNA of Alpine Permo-Triassic rock salt sediment samples, which involved centrifugation instead of ultrafiltration for enrichment of cells and several washing steps for detaching cells from sediment particles, Archaeal biodiversity was re-examined . For this purpose, a 16S rRNA gene clone library was created and archaeal sequences were characterized via phylogenetic analysis of amplified ribosomal DNA restriction analysis (ARDRA) with DdeI and 16S rDNA sequencing as well as denaturing gradient gel electrophoresis analysis (DGGE). Inserts of clones representing ten ARDRA groups were analysed with partial sequence analysis and representatives of different phylogenetic groups were sequenced double stranded. This possibility of rapid characterization of a total clone library via restriction analysis facilitated the detection of low abundance phylotypes. The 16S rRNA gene library contained 11 different phylotypes, all of which belonged to the Halobacteriaceae. Fifty-three partial haloarchaeal sequences were obtained as well as twenty total insert sequences of clones. Four novel phylotypes clustered tightly with four sequence clusters found in a previous study of Archaeal biodiversity of Alpine rock salt (Radax et al. 2001). One sequence (HW 50) had 99.0% identity to Halococcus morrhuae and another (HW 23) had 97% identity to Natronomonas pharaonis. Four phylotypes were closely related to cultured Halobacteriaceae (more than 97% similarity) and to strains isolated from salt mine brines; one phylotype was 94-94.6% similar to mainly alkaliphilic halobacteria. Six phylotypes were only remotely related to cultured Halobacteriaceae (less than 89-92% similarity), suggesting that they represent uncultured novel haloarchaeal taxa in rock salt. The novelty of the sequences suggested also avoidance of haloarchaeal contaminants during preparation of DNA and PCR reactions. The data presented here also indicate that our present view of halobacterial diversity is far from being complete.

Anbieter: Orell Fuessli CH
Stand: 18.09.2020
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60s Clustered Heritage Boots in Camel
Topseller
67,95 € *
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''These 60s Clustered Heritage Boots in Camel are made for walking... and dancing... or even shopping!'' ;-) Diese vielseitigen Stiefeletten sind der perfekte Begleiter in dieser Jahreszeit! Hergestellt aus hellbraunem Leder-Imitat mit Schnürsenkeln, geflochtenen Details und einem hohen Blockabsatz für den perfekten Vintage-Look.''Boot season is here!'' * Leder-Imitat * Runde Schuhspitze * Geflochtene Details * Hoher Blockabsatz * Schnürsenkel * Komfortables Fußbett * Anti-rutsch Sohle

Anbieter: Topvintage
Stand: 18.09.2020
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Chicago's Polish Downtown
19,99 € *
zzgl. 3,00 € Versand

Polish Downtown is Chicago's oldest Polish settlement and was the capital of American Polonia from the 1870s through the first half of the 20th century. Nearly all Polish undertakings of any consequence in the U.S. during that time either started or were directed from this part of Chicago's near northwest side. This book illustrates the first 75 years of this influential Polish neighborhood. Featured are some of the most beautiful churches in Chicago-St. Stanislaus Kostka, Holy Trinity, and St. John Cantius-stunning examples of Renaissance and Baroque Revival architecture that form part of the largest concentration of Polish parishes in Chicago. The headquarters for almost every major Polish organization in America were clustered within blocks of each other, and four Polish-language daily newspapers were published here. The heart of the photographic collection in this book is from the extensive library and archives of the Polish Museum of America, still located in the neighborhood.

Anbieter: Thalia AT
Stand: 18.09.2020
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Characterization of a haloarchaeal 16S rRNA gen...
34,99 € *
ggf. zzgl. Versand

Diploma Thesis from the year 2002 in the subject Biology - Micro- and Molecular Biology, grade: 1,0 (A), University of Salzburg (Institute for Genetics and General Biology), language: English, abstract: Using a new method to extract total community DNA of Alpine Permo-Triassic rock salt sediment samples, which involved centrifugation instead of ultrafiltration for enrichment of cells and several washing steps for detaching cells from sediment particles, Archaeal biodiversity was re-examined . For this purpose, a 16S rRNA gene clone library was created and archaeal sequences were characterized via phylogenetic analysis of amplified ribosomal DNA restriction analysis (ARDRA) with DdeI and 16S rDNA sequencing as well as denaturing gradient gel electrophoresis analysis (DGGE). Inserts of clones representing ten ARDRA groups were analysed with partial sequence analysis and representatives of different phylogenetic groups were sequenced double stranded. This possibility of rapid characterization of a total clone library via restriction analysis facilitated the detection of low abundance phylotypes. The 16S rRNA gene library contained 11 different phylotypes, all of which belonged to the Halobacteriaceae. Fifty-three partial haloarchaeal sequences were obtained as well as twenty total insert sequences of clones. Four novel phylotypes clustered tightly with four sequence clusters found in a previous study of Archaeal biodiversity of Alpine rock salt (Radax et al. 2001). One sequence (HW 50) had 99.0% identity to Halococcus morrhuae and another (HW 23) had 97% identity to Natronomonas pharaonis. Four phylotypes were closely related to cultured Halobacteriaceae (more than 97% similarity) and to strains isolated from salt mine brines; one phylotype was 94-94.6% similar to mainly alkaliphilic halobacteria. Six phylotypes were only remotely related to cultured Halobacteriaceae (less than 89-92% similarity), suggesting that they represent uncultured novel haloarchaeal taxa in rock salt. The novelty of the sequences suggested also avoidance of haloarchaeal contaminants during preparation of DNA and PCR reactions. The data presented here also indicate that our present view of halobacterial diversity is far from being complete.

Anbieter: Thalia AT
Stand: 18.09.2020
Zum Angebot